Dakota Fanning

Fern Arable

Julia Roberts

Charlotte the Spider (voice)

Steve Buscemi

Templeton the Rat (voice)

John Cleese

Samuel the Sheep (voice)

Oprah Winfrey

Gussy the Goose (voice)

Cedric the Entertainer

Golly the Goose (voice)

Reba McEntire

Betsy the Cow (voice)

Kathy Bates

Bitsy the Cow (voice)

Robert Redford

Ike the Horse (voice)

Thomas Haden Church

Brooks the Crow (voice)

André 3000

Elwyn the Crow (voice)

Dominic Scott Kay

Wilbur (voice)

Sam Shepard

Narrator (voice)

Abraham Benrubi

Uncle the Pig (voice)

Kevin Anderson

Mr. Arable

Essie Davis

Mrs. Arable

Siobhan Fallon Hogan

Mrs. Zuckerman

Louis Corbett

Avery

Robyn Arthur

Teacher

Julian O'Donnell

Henry Fussy

Gary Basaraba

Homer Zuckerman

Nate Mooney

Lurvy

Nicholas Bell

Minister

Beau Bridges

Dr. Dorian

Teague Rook

Well Dressed Man

Julia Zemiro

Bystander

Denise Kirby

New Teacher

Robert Plazek

Ball Thrower

Joseph Lotesto

Young Boy

Michael Roland

Mayor

Don Bridges

Announcer

Ian Watkin

Fair Official

Joel McCrary

Sheep Group (voice)

Brian Stepanek

Sheep Group (voice)

Fred Tatasciore

Sheep Group (voice)

Bradley White

Sheep Group (voice)

Maia Kirkpatrick

Joy the Baby Spider (voice)

Jennessa Rose

Aranea the Baby Spider (voice)

Briana Hodge

Nellie the Baby Spider (voice)

Dale Azzopardi

Photographer (uncredited)

Ella Scott Lynch

Girl at Fair (uncredited)